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Applications: Study enzyme kinetics and small screen molecule inhibitors for drug discovery and high throughput screening (HTS) applications.
Background: Aurora Kinase A, also known as serine/threonine-protein kinase 6, is part of the Aurora subfamily. Aurora Kinase A is a CPC (chromosomal passenger complex) protein. It plays a role in microtubule organization during mitosis e meiosis. It recruits several proteins required for mitotic spindle formation, such as kinesin 5 and γ-tubulin, and its interaction with the kinetochore. It has a pattern of expression and subcellular localization that varies with the cell cycle stage, in agreement with its role as cell cycle regulator. It has oncogenic activity and is overexpressed in cancer tissues like breast cancer and prostate cancer. Aurora Kinase A dysfunction contributes to the development of cancer in multiple ways, which include activating the NF-κB pathway, increasing anti-apoptotic factors (Bcl-2, B-cell lymphoma 2, and MCL-1) and increase of EMT (epithelial to mesenchymal transition) related transcription factors. The use of inhibitors for Aurora Kinase A can support recovery in cases of cancer where drug resistance has occurred. Further studies will elucidate the exact mode of action of Aurora Kinase A in cancer development and progression and open new therapeutic avenues.
Contraindications: The final concentration of DMSO in the assay should not exceed 1%.
Description: The Chemi-Verse™ Aurora Kinase A Assay Kit is a luminescence assay designed to measure Aurora Kinase A activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant Aurora Kinase A, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Uniprot: O14965
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
References: Tang A., et al., 2017 Oncotarget 8(14):23937-23954.
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