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Applications: Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.
Background: Bruton's tyrosine kinase or BTK, is a non-receptor kinase protein found in hematopoietic and plasma cells. It is involved in functionality, maturation, and apoptosis of B cells, in addition to regulating signals in the MAPK (mitogen-activated protein kinase), PI3K (phosphoinositide 3-kinase) and NF-κB (nuclear factor kappa B) pathways. The BTK pathway has implications for a number of autoimmune disorders including isolated growth hormone deficiency type III and rheumatoid arthritis. BTK inhibitors have been used in the treatment of inflammatory diseases and hematological disorders including cancer. For instance, Ibrutinib (also known as PCI-32765), is used in the clinic for the treatment of CLL (chronic lymphocytic leukemia), MCL (mantle cell lymphoma) and GvHD (graft versus host disease) and other diseases. However, low-selectivity BTK inhibitors have severe side effects, so further refinement is necessary. Development of highly selective BTK inhibitors will lead to greater benefits in the treatment of cancer and immune diseases.
Contraindications: The final concentration of DMSO in the assay should not exceed 1%.
Description: The Chemi-Verse™ BTK Kinase Assay Kit is designed to measure BTK (Bruton's tyrosine kinase) kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified BTK, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Uniprot: Q06187
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
References: Rawlings D., et al., 1993 Science 261(5519):358-361.
Alrouji M., et al., 2023 Scientific Reports 13: 17684.
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