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Applications: Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.
Background: Cyclin-dependent kinase 9 (CDK9) is the catalytic subunit of the positive transcription elongation factor b (P-TEFb) complex, which phosphorylates the C-terminal domain of RNA polymerase II, a key player in the production of mature RNA. P-TEFb also includes cyclinT or cyclinK. CDK9 translocates to the nucleus once it gets autophosphorylated, where it can associate with cyclinT or K. Hyperactivation of cyclins is known to result in cancer and the development of resistance to therapy, and CDK9-cyclinK is involved in genome integrity. Lack of CDK9 or cyclinK was shown to impair cell recovery after cells were stressed and potentiated DNA damage. Further understanding of the role of CKD9/cyclinK in health and disease and how to modulate it may prove beneficial for fields such as cancer therapy.
Contraindications: The final concentration of DMSO in the assay should not exceed 1%.
Description: The Chemi-Verse™ CDK9/CyclinK Kinase Assay Kit is designed to measure CDK9 (cyclin dependent kinase 9)/Cyclin K kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant CDK9/CyclinK complex, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Uniprot: CDK9: P50750; CyclinK: O75909
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
References: Napolitano G, et al., 2003 J Cell Physiol. 197(1):1-7.
Yu D. and Cortez D., 2011 Cell Cycle 10(1):28-32.
Li J., et al., 2022 J Med Chem. 65(16):11034-11057.
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