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Applications: Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.
Background: Aurora Kinase C, also known as serine/threonine-protein kinase 13, is part of the Aurora subfamily. Aurora Kinase C is a CPC (chromosomal passenger complex) protein and forms a complex with Aurora Kinase B and centromere proteins. It plays a role in microtubule organization during mitosis and meiosis, mostly in meiosis. It has a pattern of expression and subcellular localization that varies with the cell cycle stage, in agreement with its role in cell cycle regulation. It has oncogenic activity and is overexpressed in cancer cell lines, by performing similar functions to Aurora Kinase B and other still unknown mechanisms. Mutations that inactivate this protein result in male infertility, being the main cause of infertility in North African men. The use of inhibitors can increase the effectiveness of chemotherapy and has been shown to stop the progress and growth of several cancers. Further studies will elucidate the exact mode of action of Aurora Kinase C in cancer development and progression and open new therapeutic avenues.
Contraindications: The final concentration of DMSO in the assay should not exceed 1%.
Description: The Chemi-Verse™ Aurora Kinase C Assay Kit is a luminescence assay designed to measure Aurora Kinase C activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant Aurora Kinase C, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Uniprot: Q9UQB9
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
References: Tang A., et al., 2017 Oncotarget 8(14):23937-23954.
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