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Applications: Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.
Background: PAK3, also known as P21 protein activated kinase 3, beta-PAK, belongs to the group I of the PAK family of serine/threonine kinases. It is mostly found in neuronal cells, and it is involved in synapse formation and plasticity. Mutations in this protein can result in nonsyndromic X-linked mental retardation, and overexpression is found in neuroendocrine tumors. It has also been found at high levels in hepatocellular carcinoma (HCC), where it correlates with the stage of the disease, and in lung cancer. In non-small cell lung cancer (NSCLC), PAK3 levels seem to relate to Smad4 deficiency, as lack of Smad4 (mother against decapentaplegic homolog 4) reduces the expression of miR-495 and miR-543, which are inhibitors of PAK3 expression. PAK3 in turn activates the PAK3-JNK (c-Jun N-terminal kinase)-Jun pathway, which increases metastasis. Inhibition of PAK3 can thus be a promising tool in cancer therapy.
Contraindications: The final concentration of DMSO in the assay should not exceed 1%.
Description: The Chemi-Verse™ PAK3 Kinase Assay Kit is designed to measure PAK3 (P21 activated kinase 2, beta-PAK) serine/threonine kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant PAK3 kinase, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Uniprot: O75914
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
References: Tan X., et al., 2021 Nature Communications 12:4853.
Gao Z., et al., 2022 J Cancer 13(1):153:161.
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