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Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Background: CHK1 is a 56 kd serine/threonine protein kinase that was originally identified in fission yeast to play a role in activation of the DNA damage checkpoint in the G2 phase of the cell cycle (1). CHK1 appears to function downstream of several of the known fission yeast checkpoint gene products, including that encoded by rad3+, a gene with sequence similarity to the ATM gene mutated in patients with ataxia telangiectasia (2).
Description: Recombinant full-length human CHK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST-tag. The gene accession number is NM_001274.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.
Genbank: NM_001274
Host Cell Line: Sf9 cells
Purity: ≥95%
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: O14757
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Walworth, N. et al. Nature. 1993 May 27,363(6427):368-71.
2. Walworth, NC. et al. Science. 1996 Jan 19,271(5247):353-6.
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