Target: Choline Acetyltransferase.
Conjugate: ATTO 488.
Product Type: Polyclonal.
Immunogen: Synthetic peptide from the N-terminal to the mid-protein of human Choline O-Acetyltrasferase.
Swiss-Prot: P28329.
Purification: Peptide Affinity Purified.
Storage Buffer: PBS, 50% glycerol, 0.09% sodium azide.
Concentration: 1 mg/ml.
Specificity: Predicted molecular weight at ~82.5kDa. Observed molecular weights between 68-70kDa.
Cellular Localization: Cytoplasm, Cytosol, Mitochondrion, Nucleus.
Scientific Background: Acetylcholine (ACh) is a common neurotransmitter for motoneurons, preganglionic autonomic neurons, postganglionic parasympathetic neurons, a variety of brain regions and some emerging neuron-like stem cells. The metabolism of Ach is relatively simple, involving only two enzymes: choline acetyltransferase (ChAT) for synthesis and acetylcholinesterase (AChE) for degradation. Further, acetylcholine has little function in neurons other than neurotransmission and seems to be neuron specific. It seems that only cholinergic neurons have significant amounts of ChAT making anti-choline acetyltransferase a useful specific marker. ChAT is a valuable marker for diseases associated with decreased cholinergic function such as Schizophrenia, Alzheimer disease and Down syndrome (1-3).
References: 1. Houser C.R., Crawford G.D., Barber R.P., Salvaterra P.M., Vaughn J.E. (1983) Brain Research. 266(1): 97-119.2. Karson C.N., Casanova M.F., Kleinman J.E.m Griffin W.S. (1993) Am J Psychiatry. 150: 454-459. 3. Baskins D.S., et al. (1999) Arch Neurol. 56: 1121-1123.
Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
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