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Applications: Useful for the kinase assay kits and Western blotting.
Background: CK1α1 is a member of the CK1 family of serine/threonine protein kinases which play an important role in diverse cell processes. CK1α1 can regulate Smo cell surface accumulation and activity in response to hedgehog. CK1α1 phosphorylate Smo at several sites and phosphorylation-deficient forms of Smo fail to accumulate on the cell surface and are unable to transduce the hedgehog signal (1). CK1α1 dynamically associates with the CBM complex on T cell receptor engagement to participate in cytokine production and lymphocyte proliferation. CK1α1 can form complex with MDM2 which then regulates the stability of p53 and E2F-1 transcription factors (2).
Description: Full length recombinant human CK1 alpha 1 also known as CSNK1A1, CK1, HLCDGP1, PRO2975, CK1A1. Genbank Accession NM_001892 with an N-terminal GST-tag, expressed in Sf9 cells via a baculovirus expression system. MW = 62kDa.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Genbank: NM_0061892
Purity: ≥80%
Storage Stability: At least 6 months at -80°C. Avoid freeze/thaw cycles.
Tags: GST-Tag
Uniprot: P48729
Biosafety Level: Not applicable (BSL-1)
References: 1. Jia, J., et al., Nature 2004;432:1045-1050.
2. Huart, A.S., et al., J Biol Chem. 2009;475:284(47):32384-94.
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