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Applications: Screen and validate antibodies against Claudin-1 for drug discovery and research.Perform cell-based assays, such as screening for potential Claudin-1 ligands.
Background: Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the permeability to ions and small molecules. They are also involved in cell growth and differentiation. Often, several claudin family members are co-expressed and interact with each other, and this determines the overall cell permeability. Claudin-1 is required to prevent the paracellular diffusion of small molecules through tight junctions in the epidermis and is required for the normal barrier function of the skin. It is involved in normal water homeostasis and prevention excessive water loss through the skin, probably via an indirect effect on the expression levels of other proteins, since Claudin-1 itself seems to be dispensable for water barrier formation in keratinocyte tight junctions. The expression levels and localization of claudins relate to the ability of tumors to invade and to their aggressive characteristics. Claudin 1 is one of the most studied claudins in the context of cancer, but its precise roles are still unclear. Further studies into its roles will provide new cancer therapy options.
Description: CHO cell line stably expressing human Claudin-1 (NM_021101.5). Surface expression of Claudin-1 was confirmed by flow cytometry. The stable clonal cell line was selected for its high levels of Claudin-1 expression compared to the parental CHO-K1 cell line.
Host Cell Line: CHO-K1 cells, Chinese Hamster Ovary, epithelial-like cells, adherent
Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.
Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Supplied As: Each vial contains >1 x 106 cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)
Uniprot: O95832
Warnings: Avoid freeze/thaw cycles
Biosafety Level: BSL-1
References: Bhat A., et al., 2020, Int J Mol Sci 21(2):569.
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