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Encompassing Amino Acids: 1-220(end)
Application: Useful for binding assays and SDS-PAGE.
Assay Conditions: Binding to Anti-Claudin-6 antibody (Santa Cruz Biotechnology #sc-393671) was performed using ELISA. The Claudin-6 protein was coated onto a 96-well plate overnight at 4°C (50 µl/well at a concentration of 2 µg/ml in PBS). The plate was washed 3 times with Immuno Buffer 1 (BPS Bioscience #79311) containing DDM/CHS detergent (DDM 0.05%, CHS 0.01%), and blocked using 100 µl of Blocking Buffer 2 (BPS Bioscience #79728) with DDM/CHS for 1 hour at room temperature. After removing the blocking buffer, 50 µl/well of purified anti-Claudin-6 antibody serial dilutions in Blocking Buffer 2 with DDM/CHS detergent was added and incubated for 45 minutes at room temperature. After 3 washes, the plate was incubated with Anti-Mouse IgG H&L (HRP) (Abcam #ab7061), washed 3 times, and incubated with Colorimetric HRP substrate (BPS Bioscience #79651). The reaction was stopped, and absorbance was read at 450 nm. The Blank value was subtracted from all values.
Background: Claudins are integral membrane proteins and major components of tight junction strands which serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Claudin-6 is one of the 27 members of the claudin family. Claudin-6 is widely expressed in various tumors but rarely expressed in healthy adult tissues. This cancer specific expression of claudin-6 makes it a promising target as an antigen for cancer immunotherapy. Several therapeutics that target claudin-6 are currently in development or in clinical trials including bispecific antibodies, monoclonal antibodies, and CAR-T cell therapy products.
Description: Recombinant human claudin-6, full length encompassing amino acids 1-220 (end). This construct contains a C-terminal FLAG-tag. This protein was affinity purified.
Format: Aqueous buffer solution.
Formulation: 50 mM HEPES, pH 8.0, 300 mM NaCl, 10% Glycerol, 0.1% DDM (dodecyl-β-D-maltoside), 0.01% CHS (cholesteryl hemisuccinate), and 140 µg/ml FLAG peptide
Genbank: NM_021195.5
Host Cell Line: HEK293
Purity: ≥90%
Storage Stability: At least 6 months at -80°C.
Uniprot: P56747
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
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