Clone: VI-AP and VI-AP
Label: FITC,PE
Background: This ready to use Negative Control reagent contains a combination of fluorescein and phycoerythrin conjugated mouse immunoglobulin molecules of IgG1 isotype, which have been selected on the basis of their binding characteristics: no specific binding to human intracellular or cell surface antigens, same low range of nonspecific binding to human leukocytes as other COMBI-IC-Reagents. Like all other COMBI-IC-reagents, this reagent should be used in combination with our FIX&PERM? Cell Permeabilization Kit (Cat.No. GAS-002) These isotype control IgG1 are suitable as negative controls to be used in combination with COMBI-IC reagents for the: - Enumeration of Myeloid Cells - Analysis of Myeloid Differentiation Stage - Enumeration of B-cells and Precursors - Enumeration of T-cells and Precursors - Analysis of Leukemia Cells - Analysis of Immunodeficiency States Results must be interpreted by a certified professional before final interpretation. Analyses performed with this antibody should be paralleled by positive and negative controls. If unexpected results are obtained which cannot be attributed to differences in laboratory procedures, please contact us.
Specificity: VI-AP reacts with calf intestine alkaline phosphatase and does not show cross-reactivity with human proteins.
Formulation: PBS pH 7.2, 1% BSA, 0.05% NaN3
References: 1. J. L. Clarke, W. Watkins, J Biol Chem 271, 10317 (1996). 2. R. N. Knibbs et al., J Cell Biol 133, 911 (1996). 3. B. Kniep et al., J Biochem (Tokyo) 119, 456 (1996). 4. A. J. Wagers, L. M. Stoolman, R. Kannagi, R. Craig, G. S. Kansas, J Immunol 159, 1917 (1997). 5. M. Noguchi, N. Sato, H. Sugimori, K. Mori, K. Oshimi, Leuk Res 25, 847 (2001). 6. W. M. Watkins, J. L. Clarke, Adv Exp Med Biol 491, 231 (2001).
Caution: For professional users only. This reagent contains sodium azide. To avoid the development of hazardous conditions, reagents containing azide should be diluted in running water prior to be discarded. Similar to the work with other biological products, proper handling procedures are recommended.
English
Deutsch
