Label: FITC.
Background: The antibody identifies the inactivated C3c-component and is useful for detection of C3-deposit in tissues after complement activation. Of special interest is the analysis of C3c in different kinds of pemphigus. In this disease deposits of C3 are mainly found in the basal membrane of the skin and to some extend in the basal keratinocytes of the epidermis. C3c-component.
Positive Control: Skin (basal membrane) in bullous pemphigoid.
Immunogen: Purified Complement 3 from human serum.
Purification Method: Ion-exchanged purified IgG conjugated with Fluorescein Isothiocyanate Isomer 1 in PBS pH 7.2; contains sodium azide (0,09%)**.
Source: C3c is the major fragment resulting from C3 cleavage by C3 convertase and factor I. It is composed of an intact beta chain bound to two fragments of the alpha chain. C3c is isolated and purified from pooled normal human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity: The antiSerum does not cross-react with any other component of Human plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
References: 1. Bernard P., Aucouturier P., Denis F., and J.M. Bonnetblanc (1990) Immunoblot analysis of IgG subclasses of circulating antibodies in bullous pemphigoid. Clin. Immunol. Immunopathol. 54(3); 484-494. 2. Feliciani C., Pour S.M., Toto P., Coscione G., and Amerio P. (1998) Direct immunofluorescence diagnosis of pemphigus without biopsy. J. Cutan. Med. Surg. 2(4); 209-211. 3. Buschman K.E., Seraly M., Thong H.Y., Deng J.S., Draviam R.P., and Abernet J.L. (2002) A predominant IgG4 subclass may be responsible for True-negative direct immunofluorescence in bullous pemphigoid. J. Cutan. Pathol. 29(5); 282-286.
UniProt: P01024-CO3_HUMAN.
Caution: *These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
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