The principle of the Nucleofector™ Technology:

By applying electrical impulses, small pores are created in the cell membrane. An interplay of preprogrammed nucleofection protocols and coordinated reagents results in optimally adapted conditions for a successful transfection for the cell type used. The substrate is not only taken up by the cytoplasm, but also by the cell nucleus due to the optimized conditions. This process enables high transfection rates with an efficiency of up to 99%.

We offer the following platforms:

• For maximum flexibility: 4D Nucleofector
• For medium throughput: Add-on 96-well Shuttle
• For high throughput: HT Nucleofector
• The basic device: Nucleofector 2b

Selected products from our webshop:

You can find all available products in our webshop.

4D-Nucleofector LV Unit

 

 

The technical innovations make it possible to use the proven Nucleofector technology on a larger scale: 10⁷ to 10⁹ cells.

Transfection protocols of the X-Unit (transfections on a smaller scale) can be adopted without additional optimization. The ease of transmission of the protocols has been tested for various cell types including human T cells, CHO-S, HEK293-S, or K562.

 

 

 

 

 

 

Benefits:

• Closed system - sterile transfection of up to 10⁹ cells
• Pre-optimization on a small scale
• Established protocols - benefit from > 700 optimized cell types
• Easy to use - minimal training required
• 4D-Nucleofector™ LogWare - Optional operation via 21CFR Part 11 compliant software

Applications:

• Ex vivo modification of human primary cells for cell therapy applications (e.g. genome editing, production of CAR-T cells)
• Production of potential therapeutic proteins or antibodies for screening applications
• Generation of large numbers of transiently modified primary cells for cell-based assay

 

AMAXA NUCLEOFECTION KITS

The Nucleofector Kits contain special transfection cuvettes, Nucleofector reagents (buffer and supplement) and the control plasmid pmaxGFP. The buffer and the associated supplement are specially optimized for the respective primary cells. Nucleofector reagents provide an optimal environment and enable the highest transfection rates and cell viability, while the physiological character and functions of the cells are preserved.

Selected products from our webshop:

You can find all available products in our webshop.

 

VECTORS AND TRANSFECTION CONTROL KITS

We offer a wide range of expression vectors and transfection control kits from our supplier Lonza.

• pmaxFP™ Green, Yellow and Red Fluorescent Protein
• pmaxCloning™ vector, for cloning a specific gene
• siRNA Test Kit, for the simple establishment of siRNA transfection experiments
• Peptide Transfection Control, a fluorophore-labeled control peptide that can be used as a positive control for the nucleofection of peptides