Target: ATP7A
Conjugate: Unconjugated
Product Type: Monoclonal
Clone Number: L60/4 (Formerly sold as S60-4)
Immunogen: Synthetic peptide amino acids 42-61 (cytoplasmic C-terminus) of human Copper- transporting ATPase1
Swiss-Prot: Q04656
Purification: Protein G Purified
Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Concentration: 1 mg/ml
Specificity: Detects ~180kDa in rat brain membrane preparations.
Cellular Localization: Endoplasmic Reticulum,Cytoplasm,Golgi Apparatus,Trans-Golgi Network Membrane,Cell Membrane
Scientific Background: The copper efflux transporters ATP7A and ATP7B sequester intracellular copper into the vesicular secretory pathway for export from the cell. ATP7A (also known as Copper-transporting ATPase 1) functions as a transmembrane copper-trans locating P-type ATPase and plays a vital role in systemic copper absorption in the gut and copper reabsorption in the kidney. Polarized epithelial cells such as Madin-Darby canine kidney cells are a physiologically relevant model for systemic copper absorption and reabsorption in vivo. Although ATP7A is not detectable in most normal tissues, it is expressed in a considerable fraction of many common tumor types. Increased expression of ATP7A renders cells resistant to cisplatin and carboplatin. Mutations in the ATP7A gene result in Menkes disease, which is fatal in early childhood. Mutations in the ATP7B gene lead to the autosomal recessive disorder, Wilson disease, characterized by neurological symptoms and hepatic damage.
References: 1. Samimi G., et al. (2003) Clin. Cancer Res. 9: 5853-9.2. Samimi G., et al. (2004) Mol Pharmacol. 66: 25-32.3. Greenough M., et al. (2004) Am. J. Physiol. Cell Physiol. 287: C1463-71.4. Song, I.S., et al. (2004) Mol. Cancer Ther. 3: 1543-1549.5. van Dongen, E.M., et al. (2004) Biochem. Biophys. Res. Commun. 323: 789-795.6. Samimi, G., et al. (2004) Mol Pharmacol 66: 25-32.7. Morgan, C.T., et al. (2004) J. Biol. Chem. 279: 36363-36371.8. Barnes, N., et al. (2005) J. Biol. Chem. [Epub].
Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
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