Target: Cortisol
Product Type: EIA Kits
Trademark: StressXpress®
Detection Method: Colorimetric Assay
Assay Type: Competitive EIA (Enzyme Immunoassay)
Utility: EIA kit used to quantitatively measure cortisol present in samples.
Precision: Intra Assay Precision: Three human samples were diluted with Assay Buffer and run in replicates of 20 in an assay. The mean and precision of the calculated Cortisol concentrations were:Sample 1- 1174.3 pg/mL, 6% CVSample 2- 475.9 pg/mL, 5.6% CVSample 3- 177.4 pg/mL, 14.7% CVInter Assay Precision: Three human samples were diluted with Assay Buffer and run in duplicates in ten assays run over multiple days by four operators. The mean and precision of the calculated Cortisol concentrations were:Sample 1- 1188.1 pg/mL, 7.2% CVSample 2- 508.7 pg/mL, 6.3% CVSample 3- 199.7 pg/mL, 10.9% CV
Sample Type(s): Dried Fecal Samples,Saliva,Urine,Serum,EDTA Plasma,Heparin Plasma,Tissue Culture Media
Number of Samples: 40 samples in duplicate
Incubation Time: 90 Minutes
Sensitivity: 17.3 pg/ml
Assay Range: 100 - 3200 pg/ml
Scientific Background: Cortisol, C21H30O5, (hydrocortisone, compound F) is the primary glucocorticoid produced and secreted by the adrenal cortex. It is often referred to as the “stress hormone” as it is involved in the response to stress and it affects blood pressure, blood sugar levels, and other actions of stress adaptation. Immunologically, cortisol functions as an important anti-inflammatory and plays a role in hypersensitivity, immunosuppression, and disease resistance (1). In the metabolic aspect, cortisol promotes gluconeogenesis, liver glycogen deposition, and the reduction of glucose utilization (2). Production of cortisol follows an ACTH-dependent circadian rhythm, with a peak level in the morning and decreasing levels throughout the day. Most serum cortisol, all but about 4%, is bound to proteins including corticosteroid binding globulin and serum albumin (1,3). Only free cortisol is available to most receptors and it is through these receptors that physiological processes are modulated. Abnormal cortisol levels are being evaluated for correlation with a variety of different conditions, such as prostate cancer (4), depression (5), and schizophrenia (6). It is already known that abnormal levels of cortisol are involved in Cushing's Syndrome and Addision's disease (7).
References: 1. E. Friess, et al., Eur J Clin Invest, 2000, 30, Suppl 3:46-50.2. Freeman, Scott, 2002. Biological Science. Prentice Hall; 2nd Pkg edition (December 30, 2004).3. C. Longscope., J. Endocrinology, 1996, , Suppl S125-S127.4. J. Herbert, Lancet, 1995 345, 1193-1194.5. A. Michael, et al., Biol. Psychiatry, 2000, 48, 989-95.6. C.R. Dequet and D.J. Wallace, Current Opin. Ivest. Drugs, 2001, 8, 1045-53.7. W.M. Jeffries, Med. Hypotheses, 1998, 51, 114-4.
Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
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