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Encompassing Amino Acids: 1-604 (end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Mixed various concentrations of enzyme in Assay buffer (100 mM Tris-HCl pH 8, 3 uM EDTA) with 5 µM Hematin for 10 min then added 100 M Ampliflu Red and 200 µM arachidonic acid. Fluorescence (ex. 535 em. 590) was measured after 5 minutes at room temperature. Reaction volume was 100 µl.
Description: Human COX2, GenBank Accession No. NM_000963, a.a. 1-604 (end), with C-terminal FLAG-His-tag, MW=71 kDa, expressed in a baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 80 µg/ml FLAG peptide, 300 µM diethyldithiocarbamate and 0.08% Triton X-100.
Genbank: NM_000963
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal FLAG-His-tags
Uniprot: P35354
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Kosaka, T., et al., Eur J Biochem. 1994 May 1;221(3):889-97.
2. Cox, D.G., et al., Br J Cancer. 2004 Jul 19;91(2):339-43.
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