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Encompassing Amino Acids: CRBN: 2-442(end); DDB1: 2-1140(end)
Application: Useful for SDS-PAGE.
Background: The Cereblon (CRBN) protein via its interaction with the DNA damage-binding protein-1 (DDB1), Cullin 4 (Cul4A or Cul4B), and regulator of Cullins 1 (RoC1) forms a functional E3 Ub ligase complex. In this complex, Cereblon functions as a substrate receptor that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Like most E3 ligases, Cereblon complex ubiquitinates itself and its auto-ubiquitination promotes its ligase activity. Cereblon complex is involved in many biological processes including cell proliferation and apoptosis, and it has been extensively used in targeted protein degradation (PROTAC®), where CRBN binds to the protein of interest and leads to its degradation. This approach has shown promising results in the treatment of cancer.
Description: Complex of recombinant human CRBN (Cereblon), encompassing amino acids 2-442(end), and recombinant human DDB1 (damage specific DNA binding protein 1), encompassing amino acids 2-1140(end). The CRBN and DDB1 constructs contain an N-terminal FLAG-tag. These recombinant proteins were co-expressed, and affinity purified.
Format: Aqueous buffer solution.
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Genbank: CRBN: NM_016302; DDB1: NM_001923
Host Cell Line: HEK293
Purity: ≥90%
Storage Stability: At least 6 months at -80°C.
Tags: N-Terminal FLAG Tag
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
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