CRE/CREB Reporter Kit (cAMP/PKA Signaling Pathway)

Products from BPS Bioscience require a minimum order value above 400€

Applications: Monitor the JNK signaling pathway activity and AP1-mediated activity.
Screen for activators or inhibitors of the JNK signaling pathway.
Study effects of RNAi or gene overexpression on the activity of the JNK pathway.

Concentration: Both the Reporter and Negative Control Reporter are provided at 60 ng DNA/µl (500 µl each)

Description: The CRE/CREB Reporter kit is designed for monitoring the activity of the cAMP/PKA signaling pathway in cultured cells. The kit contains transfection-ready CRE luciferase reporter. This reporter contains the firefly luciferase gene under the control of multimerized cAMP response element (CRE) located upstream of a minimal promoter. Elevation of the intracellular cAMP level activates cAMP response element binding protein (CREB) to bind CRE and induces the expression of luciferase. The CRE reporter is premixed with constitutively-expressing Renilla (sea pansy) luciferase vector that serves as an internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with the constitutivelyexpressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains the firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The negative control is critical to determining pathway-specific effects and background luciferase activity.

Format: assay kit

Storage Stability: Stable at least 12 months from date of receipt, when stored as directed (-20°C)

Supplied As: Reporter: CRE luciferase reporter vector + constitutively-expressing Renilla luciferase vector
Negative Control Reporter: Non-inducible luciferase vector + constitutively-expressing Renilla luciferase vector

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Montminy, M.R. et al. (1987) Binding of a nuclear protein to the cyclic-AMP response element of the somatostatin gene. Nature 328(6126):175-178
2. Fan Chung, K. (2006) Phosphodiesterase inhibitors in airways disease. Eur. J. Pharmacol. 533(1-3):110-117
3. Malik, R. et al. (2008) Cloning, stable expression of human phosphodiesterase 7A and development of an assay for screening of PDE7 selective inhibitors. Appl. Microbiol. Biotechnol. 77 (5): 1167-1173

• SKU: BPS60611
• Manufacturer: BPS Bioscience
• Manufacturer SKU: 60611
• Green Labware: No
• Package Unit: 500 rxns.
• Quantity Unit: PAK