Application Note: Anti-CREB-1 Antibody has been tested in western blot and suitable in ELISA assays. Specific conditions for reactivity should be optimized by the end user.
Concentration Value: 2.56 mg/ml
ELISA Dilution: 1:5,000 - 1:25,000
Gel Shift Dilution: 0.5 to 1.0 uL per assay
Western Blot Dilution: 1:500 - 1:1,000
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen: CREB-1 (p43) peptide corresponding to a region near the N-terminus of the human protein, conjugated to Keyhole Limpet Hemocyanin (KLH).
Physical State: Liquid (sterile filtered)
Purity and Specificity: Anti-CREB-1 was prepared from monospecific antiserum by delipidation and immunoadsorption against an E.coli lysate immobilized on agarose beads. Anti-CREB-1 (p43) may react non-specifically with other proteins. A partial cross-reactivity is observed against CREM-1 protein.
Background: Anti CREB-1 Antibody recognizes CREB (cAMP response element-binding), a cellular transcription factor. CREB binds to certain DNA sequences called cAMP response elements (CRE), thereby increasing or decreasing the transcription of the downstream genes. CREB was first described in 1987 as a cAMP-responsive transcription factor regulating the somatostatin gene. Genes whose transcription is regulated by CREB include: c-fos, the neurotrophin BDNF (Brain-derived neurotrophic factor), tyrosine hydroxylase, and many neuropeptides (such as somatostatin, enkephalin, VGF, and corticotropin-releasing hormone). CREB is closely related in structure and function to CREM (cAMP response element modulator) and ATF-1 (activating transcription factor-1) proteins. CREB proteins are expressed in many animals, including humans. CREB has a well-documented role in neuronal plasticity and long-term memory formation in the brain.
Low Endotoxin: No
Other: User Optimized
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