Target: Crotonaldehyde
Conjugate: ATTO 390
Product Type: Monoclonal
Clone Number: 2A8.1
Immunogen: Crotonaldehyde modified Keyhole Limpet Hemocyanin (KLH)
Purification: Protein G Purified
Storage Buffer: PBS pH 7.4, 50% glycerol, 0.09% Sodium azide *Storage buffer may change when conjugated
Concentration: 1 mg/ml
Specificity: Specific for Crotonaldehyde modified proteins. Does not cross-react with Acrolein, Hexanoyl Lysine, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, Malondialdehyde, or Methylglyoxal modified proteins.
Scientific Background: Lipid peroxidation occurs when oxidizing agents attack carbon-carbon double bonds found in unsaturated lipids. In addition to membrane degradation, oxidation end-products have been found to damage cell viability through their mutagenic and toxic properties. These downstream functional consequences facilitate the development of disease and premature aging. Crotonaldehyde (CRA) is a carcinogenic, conjugated aldehyde produced endogenously via lipid peroxidation. It covalently modifies biomolecules, forming adducts with DNA and nucleophilic amino acid residues of proteins such as lysine (1).
References: 1. Ichihashi, K. et al. (2001) J Biol Chem. 276, 23903-23913.
Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
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