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Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Variable amounts of EiCsm6 RNase activity were measured using fluorogenic substrate and read overtime using Tecan plate reader.
Description: Recombinant Enteroccous italicus EiCsm6 (type III-A CRISPR-associated CARF protein Csm6), full length. This construct contains a C-terminal His-tag. EiCsm6 is an ancillary ribonuclease associated with type III CRISPR-Cas system and contains a CRISPR-associated Rossman fold domain.
Format: Aqueous buffer solution.
Formulation: 50 mM sodium phosphate, pH 7.5, 300 mM NaCl, 200 mM imidazole, 1 mM DTT, and 10% glycerol
Genbank: WP_007208953
Purity: ≥90%
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal His-tag
Warnings: Avoid multiple freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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