Products from BPS Bioscience require a minimum order value above 400€
Applications: Great for screening small molecular inhibitors for drug discovery and HTS applications.
Description: The CTLA4:B7-1 TR-FRET Assay is designed to measure the inhibition of CTLA4 binding to B7-1 in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing biotinylated CTLA4, B7-1 protein, anti-His Tb donor, dye-labeled acceptor, and an inhibitor is incubated for two hours. Then, the fluorescence intensity is measured using a fluorescence reader.
Storage Stability: Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Huxley, P., et al. Chem Biol. 2004 Dec;11(12):1651-8.
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