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Encompassing Amino Acids: CUL3: 2-768(end); Rnx1: 1-108(end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Description: Complex of human cullin-3 (CUL3) with human RING box protein 1 (Rbx1), also known as E3 ubiquitin-protein ligase RBX1. CUL3 (a.a. 2-768(end) with N-terminal GSTtag, 117 kDa) is co-expressed with Rbx1 (a.a. 1-108(end), 12 kDa) in a baculovirus infected Sf9 cell expression system. GenBank Accession Nos. are NM_003590 (CUL3) and NM_014248 (Rbx1).
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol, and 1.6 mM glutathione
Genbank: CUL3: NM_003590; Rbx1: NM_014248
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: CUL3: Q13618; Rbx1: P62877
Warnings: Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended. If necessary, use carrier protein (BSA 0.1 - 0.5%).
Biosafety Level: Not applicable (BSL-1)
References: 1. Kaspar, J. et al., J. Biol. Chem. 2010; 285: 21349-213582. Kaspar, J. et al., J. Cell. Sci. 2012; 125: 1027-1038.
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