Application Note: Anti-Cyclin-E antibody is suitable for the detection by immunoblot, ELISA and IP. Researchers should determine optimal titers for applications that are not stated below. Antiserum will specifically react with a 45 kDa Cyclin E protein from human tissue.
Concentration Value: 70 mg/mL
ELISA Dilution: 1:100,000
Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:2,000 - 1:10,000
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen: Anti-Cyclin-E Antibody was produced by repeated immunizations with a KLH conjugated peptide corresponding to a region near the carboxy terminus of human Cyclin E.
Physical State: Liquid (sterile filtered)
Purity and Specificity: Anti-Cyclin-E was prepared from monospecific antiserum by delipidation and defibrination. No reaction was observed against other related cyclins. Cross reactivity with Cyclin E from other species may also occur.
Background: Anti-Cyclin-E antibody detects cyclin-E. Cyclin E is a member of the cyclin family. Cyclin E binds to the G1 phase Cdk2, which is required for the transition from G1 to S phase of the cell cycle that determines cell division. The Cyclin E/CDK2 complex phosphorylates p27Kip1, tagging it for degradation, thus promoting expression of Cyclin A, allowing progression to S phase. Anti-cyclin-e Antibody is ideal for investigators involved in Cell Signaling, cell biology and Signal Transduction research.
Low Endotoxin: No
Other: User Optimized
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