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Encompassing Amino Acids: 2-925
Applications: Useful as a positive control for Western blot studies.
Description: Human DDX58 protein, also known as DEAD box protein 58, retinoic acid inducible gene I, and RIG-1, GenBank Accession No. NM_014314, a.a. 2-925 (end), with N-terminal FLAG-tag, MW=108 kDa, expressed in a HEK293 expression system.
Format: Aqueous buffer solution.
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 3mM DTT, 100 µg/ml flag peptide.
Genbank: NM_014314
Purification:
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal FLAG-tag
Uniprot: O95786
Warnings: Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended. If necessary, use carrier protein (BSA 0.1 - 0.5%).
Biosafety Level: Not applicable (BSL-1)
References: 1. Jang, M., et al., Cell. 2015; 96(2): 266274.2. Suarez-Calvet, X., et al., Journal of Pathology. 2014; 233(3): 258-268.
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