iLite® CD3 Effector Assay Ready Cells are human engineered cells (Jurkat, ATCC #TIB-152). The CD3 Effector Assay Ready cells can be used alone or in combination with a target cell to measure a biological response when the NFAT pathway is induced by activation of the CD3 receptor on the effector cell, resulting in the reporter luciferase expression.
Normalization of cell counts, serum matrix effects or lysis of the effector cells by the target cells is obtained by a second reporter gene, a Renilla Luciferase reporter gene construct, under control of a constitutive promotor.