Target: Dicer
Conjugate: ATTO 488
Product Type: Monoclonal
Clone Number: N167/7 (Formerly sold as S167-7)
Immunogen: Fusion protein amino acids 1638-1899 of mouse Endoribonuclease Dicer
Swiss-Prot: Q8R418
Purification: Protein G Purified
Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Concentration: 1 mg/ml
Specificity: Detects ~215kDa.
Cellular Localization: Cytoplasm
Scientific Background: Dicer is a member of the RNase III family that specifically cleaves double-stranded RNAs to generate microRNAs (miRNAs) (1). After long primary transcript pri-miRNAs are processed to stem-looped pre-miRNAs by Drosha (2), pre-miRNAs are transported to the cytoplasm and further processed by Dicer to produce 22-nucleotide mature miRNAs (3). The mature miRNA then becomes a part of the RNA-Induced Silencing Complex (RISC) and can bind to the 3' UTR of the target mRNA (3)
References: 1. Hutvágner G. and Zamore P.D. (2002) Science 297: 2056-60.2. Lee Y., et al. (2003) Nature 425: 415-9.3. Diederichs S. and Haber, D.A. (2007) Cell 131: 1097-108.
Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
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