Dios-Arg (trifluoroacetate salt), CAS 1807353-31-6

Formulation: A solution in ethanol

Formal Name: (3β,25R)-3-[6-[[(2S)-2-amino-5-[(aminoiminomethyl)amino]-1-oxopentyl]amino]hexanoate] spirost-5-en-3-ol, bis(2,2,2-trifluoroacetate)

Purity: ≥95%

Formula Markup: C39H67N5O5 • 2CF3COO

Formula Weight: 912

Shelf life (days): 730

CAS Number: 1807353-31-6

Notes: Dios-Arg is a steroid-based cationic lipid that contains a diosgenin skeleton coupled to an L-arginine head group.{47130} It forms a complex with plasmid DNA and decreases plasmid DNA migration in an agarose-gel retardant assay at charge ratio greater than or equal to 4.{44005} Dios-Arg facilitates transfection of plasmid DNA into H1299 and HeLa cells in the presence and absence of fetal bovine serum, an effect that is reversed by the lipid raft-mediated endocytosis inhibitor methyl-β-cyclodextrin (Item No. 21633) and the caveolae-mediated endocytosis inhibitor genistein (Item No. 10005167). It has been used, coupled to 1,2-dioleoyl-sn-glycero-3-PE (DOPE; Item No. 15091), to bind siRNA and plasmid DNA to for cationic lipid nanoparticles (LNPs) for intracellular transport. Dios-Arg is cytotoxic to H1299 and HeLa cells (IC50s = 83.5 and 74.1 µg/ml, respectively).{44005}

• SKU: CAY25945-5
• Manufacturer: Cayman Chemical
• Manufacturer SKU: 25945-5
• Package Unit: 5 mg
• Quantity Unit: STK