Products from BPS Bioscience require a minimum order value above 400€
Application: Expression of human DLL1 in cells of interest.Generation of cell pools or stable cell lines expressing human DLL1 following puromycin selection.
Background: DLL1 (delta like canonical notch ligand 1) is involved in cell-to-cell communication and in embryonic development, neurogenesis, and tissue homeostasis. It is a member of the Delta-Serrate-Lag2 (DSL) family of ligands that activate the Notch signaling pathway. DLL1 binds to the Notch receptors on neighboring cells, triggering signals that regulate cell fate determination, proliferation, and differentiation. Binding of DLL1 to its receptor triggers the proteolysis of the NCID (notch intracellular domain) by ADAM10/17 (A disintegrin and metalloproteinase 10/17) and γ-secretase, and translocation to the nucleus, where it regulates the transcription of target genes. DLL1 is overexpressed in breast cancer and links to a poor prognosis by promoting cancer cell proliferation, migration, survival, and development of angiogenesis. DLL1-positive cells share similarities with CSC (cancer stem cells), being able to promote metastasis and chemoresistance. The use of Dl1.72, a monoclonal DLL1 antibody, impaired tumor growth and metastasis in an ER (estrogen receptor)-positive breast cancer xenograft model. The development of therapeutic strategies to target DLL1, alone or in combinatory therapy, will open new avenues in cancer therapy.
Description: DLL1 Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. These particles transduce cells with human DLL1 (delta like canonical notch ligand 1) (NM_005618.4), driven by a CMV promoter, and a puromycin selection marker.
Formulation: The lentivirus particles were produced in HEK293T cells in medium containing 90% DMEM + 10% FBS. Virus particles can be packaged in custom formulations by special request, for an additional fee.
Storage Stability: Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C for up to 12 months. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle.
Supplied As: Two vials (500 µl x 2) of lentivirus at a titer ≥107 TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.
Uniprot: O00548
Warnings: Avoid freeze/thaw cycles
Biosafety Level: BSL-2
References: Silva G., et al., 2021 Cancers (Basel) 13(16): 4074.
English
Deutsch
