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Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Background: DMPK (or Myotonic Dystrophy Protein Kinase) is a serine-threonine kinase that is closely related to other kinases that interact with members of the Rho family of small GTPases. DMPK protein has a leucine-rich domain at the N-terminus, a serine/threonine kinase domain in the middle, and a hydrophobic region at the C-terminus. The N-terminus of DMPK plays an important role in DMPK kinase activity, and the C-terminus of DMPK determines the intracellular localization of the protein (1). Myotonic dystrophy type 1 is an autosomal dominant disease caused by a trinucleotide repeat-expansion, cytosine-thymine-guanine (CTG)n, in the 3' untranslated region of DMPK (2).
Description: Recombinant human DMPK, full length, with N-terminal GST-tag, expressed by Sf9 insect cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.
Genbank: NM_004409
Host Cell Line: Sf9 cells
Purity: ≥80%
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q09013
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Sasagawa, N. et al: Overexpression of human myotonic dystrophy protein kinase in Schizosaccharomyces pombe induces an abnormal polarized and swollen cell morphology. J Biochem. 2003 Oct;134(4):537-42.
2. Salvatori, S. et al: Decreased expression of DMPK: correlation with CTG repeat expansion and fibre type composition in myotonic dystrophy type 1. Neurol Sci. 2005 Oct;26(4):235-42.
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