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Applications: Useful for DNA synthesis and DNA repair, as an antigen for Western blotting, and for inhibitor screening.
Background: DNA polymerase involved in base excision and repair. Has 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity that removes the 5' sugar phosphate and also acts as a DNA polymerase that adds one nucleotide to the 3' end of the arising single-nucleotide gap. Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. The protein is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage.
Description: Human DNA polymerase β (beta), also known as Pol beta and POLB (GenBank Accession No. NM_002690), a.a. 2-335 (end), with N-terminal His-tag, MW=39 kDa, expressed in an E. coli cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 250 mM imidazole, 0.04% Tween-20, and 20% glycerol.
Genbank: NM_002690
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: P06746
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Yamtich, J., et al., PLoS Genet. 2012,8(11): E1003052.
2. Wang, K., et al., Cancer Res. 1992,52(17): 4824-4827.
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