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Encompassing Amino Acids: 1792-2590
Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay was performed according to DNA Polymerase θ Assay Kit (BPS Bioscience #82107) with various amounts of DNA Polymerase θ (POLQ) protein.
Background: Polymerases are the enzymes responsible for synthesizing nucleic acids. DNA Polymerase θ, also known as DNA polymerase subunit theta, POLQ, belongs to the Family A of DNA polymerases. In addition to its DNA polymerase activity, it acts on the theta-mediated end joining (TMEJ), which classifies it as a specialized polymerase. TMEJ is a DNA repair mode that is prone to errors, and in addition to DNA polymerase θ it involves PARP1 (poly polymerase 1) and DNA ligase III. Inhibition of DNA polymerase θ in cells containing a homologous recombination (HR) deficiency, as in BRCA (breast cancer type 1/2 susceptibility protein) deficient cells, is synthetic lethal. Therefore, polymerase θ inhibitors are promising candidates for the treatment of HR-deficient cancers or in combination with HR inhibitors such as PARP inhibitors. Further development of DNA polymerase θ inhibitors will bring new therapy options to cancer patients.
Description: Recombinant human DNA polymerase θ (Theta), also known as POLQ, encompassing amino acids 1792 to 2590. This construct contains an N-terminal His-tag (6xHis) followed by a thrombin cleavage site and a SUMO-tag. This protein was affinity purified.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Genbank: NM_199420.4
Storage Stability: At least 6 months at -80°C
Tags: N-terminal His-Tag
Uniprot: O75417
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
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