Target: DNMT1
Conjugate: PerCP
Product Type: Monoclonal
Clone Number: 4G11-C7
Immunogen: Raised against a partial protein corresponding to amino acids 620-950 of human DNMT1
Swiss-Prot: P26358
Purification: Protein G Purified
Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Concentration: 1 mg/ml
Specificity: Detects ~180kDa. It will cross-react with mouse DNMT1.
Cellular Localization: Nucleus
Scientific Background: Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2F-binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells (1-6).
References: 1. Bestor T., et al.(1988) J.Mol. Biol. 203: 971-983.2. Yen R.W., Vertino P.M., Nelkin B.D., et al. (1992) Nucl. Acids Res. 20: 2287-2291.3. Xie S., et al. (1999) Gene 236: 87-95.4. Okano M., Bell D.W., Haber D.A. and Li E. (1999) Cell 99: 247-257.5. Reik W. et al. (1999) J. Nat. Genet 23: 380-382.6. Robertson K.D., et al. (2000) Nat Genet 25(3): 338-342.
Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
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