Products from BPS Bioscience require a minimum order value above 400€
Encompassing Amino Acids: 2-1632
Applications: Useful for the study of DNA methylation, screening inhibitors, and selectivity profiling.
Assay Conditions: Assayed using BPS Bioscience DNMT3A Assay Kit, Cat. #52033. Add 50 µl reaction mixcontaining DNMT3A/DNMT3L to wells coatedwith DNMT substrate. Incubate 2 hr at 37°C.Add antibody against 5-methylcytosine, thenHRP-labeled secondary antibody followed bychemiluminescent detection.
Description: Complex of active human DNA methyl-transferase 3A (DNMT3A), GenBank Accession No. NM_175629, a.a 623-912(end) with N-terminal His-tag, MW=34 kDa, and DNMT3L (GenBank Accession No. NM_013369), a.a. 160-387(end), with an N-terminal GST-tag, MW = 52 kDa, co-expressed in a baculovirus-infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 16 mM glutathione and 20% glycerol.
Genbank: NM_175629(DNMT3A), NM_013369 (DNMT3L)
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His, N-terminal GST-tag
Uniprot: Q9Y6K1 (DNMT3A), Q9UJW3 (DNMT3L)
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
English
Deutsch
