Products from BPS Bioscience require a minimum order value above 400€
Applications: Useful for studying enzyme kinetics and HTS applications.
Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt
Description: The DNMT3B Direct Activity Assay Kit is designed to measure DNMT3B activity for screening and profiling applications. The key to the DNMT3B Direct Activity Assay Kit is a highly specific antibody that recognizes 5-methylcytosine of the substrate. With this kit, only three simple steps on a microtiter plate are required for detection of DNMT3B activity. First, S-adenosylmethionine is incubated with a sample containing assay buffer and DNMT3B/3L for two hours. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
Storage Stability: 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Supplied As: Kit comes in a convenient format, with a 96-well plate precoated with DNMT substrate, an antibody against 5-methylcytosine, a secondary HRP-labeled antibody, S-adenosylmethionine, DNMT assay buffer, and purified DNMT3B/3L complex for 100 enzyme reactions.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Svedruzic, Z.M. Curr Med Chem. 2008; 15(1) :92-106.
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