Clone: Polyclonal
Background: The reactivity of the antiserum is directed to the Fc and Fab subunits of both subclasses, IgG1 and IgG2. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. In immunoelectrophoresis against goat serum a single characteristic precipitin line with a spur is usually obtained, representing primarily IgG2. If the IgG1 level in the sample approaches the concentration of IgG2, it may become visible as a second precipitin line. It does not react with any non-Ig protein in goat serum, as tested by immunoelectrophoresis and double radial immunodiffusion. As unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in nonisotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
Source: Purified normal IgG isolated from pooled goat serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity: Purified IgG fraction of polyclonal Donkey antiSerum to Goat IgG, heavy and light chains
Formulation: Purified hyperimmune donkey IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added.
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
English
Deutsch
