Application Note: Anti-DsbA-L Antibody has been tested by western blot and IHC and is suitable for usage in ELISA. Specific conditions for reactivity should be optimized by the end user. It is suggested that you add 1% BSA to the antibody dilution buffer. Anti-DsbA-L is specific for the ~25 kDa DsbA-L protein in Western blots of 3T3-L1 adipocytes and mouse white and brown adipose tissue lysate samples.
Concentration Value: Titrated value sufficient to run approximately 10 mini blots.
ELISA Dilution: 1:20,000
Immunohistochemistry Dilution: 1:200
Western Blot Dilution: 1:2000
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen: Anti-DsbA-L Antibody was produced in rabbit by repeated immunizations with full length recombinant mouse DsbA-L protein
Physical State: Liquid
Purity and Specificity: Anti-DsbA-L antibody is directed against DsbA-L. Anti-DsbA-L antibody is neat antiserum prepared from monospecific antiserum by delipidation and defibrination. Reactivity against DsbA-L occurs from mouse and human sources. Reactivity against DsbA-L from other sources is unknown.
Background: DsbA-L Antibody detects Disulfide-bond-A oxidoreductase-like protein (DsbA-L, previously named as GST Kappa) which is an adiponectin-interacting protein. DsbA-L is highly expressed in adipose tissue, and its expression level is negatively correlated with obesity in mice and humans. DsbA-L expression in 3T3-L1 adipocytes is stimulated by the insulin sensitizer rosiglitazone and inhibited by the inflammatory cytokine TNFalpha. Polymorphism of DsbA-L gene has recently been implicated in insulin secretion and body fat distribution. Overexpression of DsbA-L promotes adiponectin multimerization while suppressing DsbA-L expression by RNAi markedly and selectively reduces adiponectin levels and secretion in 3T3-L1 adipocytes. Recent studies identify DsbA-L as a key regulator for adiponectin biosynthesis. Anti-DsbA-L Antibody is ideal for investigators involved in Cell Signaling and Cell Metabolism.
Low Endotoxin: No
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