Features: Can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry. Highly purified and possess very low non-specific binding properties. Extremely high affinity for biotin. Advantages of DyLight® dyes include greater photostability, pH independence, and brighter fluorescence. Excitation at 493 nm. Emission at 518 nm. Color: Green.