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Applications: Specific biotin labeling of proteins/peptides containing the AviTag™ sequence for downstream applications.
Background: Biotin-labeling is commonly used for non-radioactive labeling and purification of proteins or other target molecules. Biotin labeling takes advantage of the exceptionally strong interaction between biotin (vitamin H) with avidin or streptavidin. The affinity of biotin to avidin or streptavidin is one of the strongest known non-covalent interactions known between a protein and a ligand, exhibiting a dissociation constant (Kd) around 4x10-14 M. BirA, an E. coli biotin protein ligase, covalently adds biotin to an AviTag™ peptide in a highly efficient and specific manner, in a reaction that requires ATP. It has the advantage of resulting in a homogeneous product that can be used in protein capture, immobilization and functionalization.
Description: The E. coli in vivo Biotinylation Kit (without BirA Competent Cells) comes in a convenient, easy-to-use format and contains the components necessary to label biotin-acceptor peptides with biotin expressed in E. coli. strain BL21, a chemically competent E. coli B strain. This kit contains sufficient reagents to label 10 x 1 L of E. coli culture. BirA-Transformed chemically competent E. coli cells (BPS Bioscience #27462) are not provided with this kit and must be purchased separately.
Storage Stability: This assay kit will perform optimally for up to 12 months from date of receipt when the materials are stored as directed.
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
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