Usage: This kit is for detection of IFN-γ, IL-2, IL-4, IL-6, IL-10, IL-17A and TNF-α in human serum, plasma, biological fluid and cell culture supernatant.
Tested Sample Types: Serum, EDTA plasma ,cell culture supernatants and other biological fluids
Assay Time: 3h
Sensitivity: TNF-α<1pg/mLIL-2<3pg/mLIFN-γ<3pg/mLIL-4<1pg/mLIL-6<5pg/mLIL-17A<3pg/mLIL-10<2pg/mL
UNIProt ID: TNF-α:P01375IL-2:P60568IFN-γ:P01579IL-4:P05112IL-6:P05231IL-17A:Q16552IL-10:P22301
Test Principle: EcoPlexTM CBA kits utilize antibodies-conjugated beads which are different in size and mean fluorescent intensity of labeling dye to capture antigens in sample. After capture beads and sample incubation, biotin-conjugated detection antibodies are added to react with antigen-beads complexes, followed by a streptavidin-R-phycoerythrin (SA-PE) treatment. The fluorescent intensity of PE on each bead populations is quantified by flow cytometer with either a single 488nm laser or dual 488nm and 633/640nm lasers. The maximum emission of the bead classification dye is at 700nm. The concentrations of protein interested are determined by comparing the fluorescent intensities to those of standard curve, like sandwich ELISA data analysis.
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