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Encompassing Amino Acids: 971- 1023
Application: Useful for cell culture and for the study of signaling pathways. Also useful for receptor binding studies, screening inhibitors, and selectivity profiling.
Biological Activity: ED50 is < 0.1 ng/ml. ED50 was determined by a cell proliferation assay using BALB/c 3T3 cells
Description: Human Epidermal Growth Factor (GenBank Accession No. NM_001963), a.a. 971- 1023, MW=6.2 kDa, expressed in an E. coli expression system.
Endotoxin Level: < 1EU per µg of EGF as determined by the LAL method.
Format: lyophilized protein
Formulation: Lyophilized from a sterile filtered solution containing 10 mM Phosphate Buffered Saline (PBS), pH 7.4, 138 mM NaCl, and 2.7 mM KCl.
Genbank: NM_001963
Purity: ≥98% by SDS-PAGE and HPLC analysis.
Reconstitution: Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
Storage Stability: ≥2 years (lyophilized) at -20°C.
Tags: none
Uniprot: P01133
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Su, X., et al. J. Biol. Chem. 282 (29),21278-21284 (2007)
2. Jin, G., et al. Cancer Sci. 98 (6), 864-868 (2007)
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