eGFP/Firefly Luciferase MM.1S Cell Line

eGFP/Firefly Luciferase MM.1S Cell Line
SKU
BPS78376
Packaging Unit
2 vials
Manufacturer
BPS Bioscience

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Applications: Use as a control for killing assaysSuitable as a B-cell target for CAR-T or CAR-NK cells for optimizing CAR design

Background: MM.1S are multiple myeloma human B lymphoblasts. The parent cell line MM.1 was established from the peripheral blood of a multiple myeloma patient who had become resistant to steroid-based therapy. MM.1S cells are sensitive to dexamethasone.The cells were infected with an integrating Firefly luciferase-eGFP lentivirus (BPS Bioscience #79980-G) and cloned by limited dilution to get a monoclonal population. The cells stably express both the Firefly luciferase and enhanced GFP genes, making these cells an excellent tool to measure specific killing by CAR-T or NK cells.

Description: Recombinant stable MM.1S cell line constitutively expressing a firefly luciferase and enhanced GFP (eGFP) cassette  driven by a CMV promoter.

Host Cell Line: MM.1S

Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.

Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Supplied As: Each vial contains 2 x 106 cells in 1 ml of cell freezing medium (BPS Bioscience, #79796)

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: BSL-1
More Information
SKU BPS78376
Manufacturer BPS Bioscience
Manufacturer SKU 78376
Green Labware No
Package Unit 2 vials
Quantity Unit PAK
Host Human
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