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Applications: In vitro and in vivo bioluminescence imaging (BLI) and fluorescence imaging.Use as target cells in CAR-T or NK co-culture killing assays.
Background: THP-1 is a human monocytic leukemia cell line, derived from the peripheral blood of a 1-year-old male with acute monocytic leukemia. THP-1 cells have Fc and C3b receptors and lack surface and cytoplasmic immunoglobulins. THP-1 cell line provides a valuable model for studying the mechanisms involved in macrophage differentiation, and for exploring the regulation of macrophage-specific genes as they relate to physiological functions displayed by these cells.
Description: eGFP/Firefly Luciferase THP-1 Cell Line is a THP-1 cell line expressing firefly luciferase and enhanced GFP (eGFP) driven by an EF1a promoter. The cells were generated by transduction with Firefly Luciferase-eGFP lentivirus (BPS Bioscience #78741), which is a SIN (self-inactivating) lentivirus.
Host Cell Line: THP-1 cells, human monocytic, leukemia, suspension
Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.
Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. Contact technical support at support@bpsbioscience.com if the cells are not frozen in dry ice upon arrival.
Supplied As: Each vial contains ~1 x 106 cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)
Warnings: Avoid freeze/thaw cycles
Biosafety Level: BSL-1
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