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Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Background: EGFR is the receptor for members of the EGF family and is a transmembrane glycoprotein that has tyrosine kinase activity. Binding of epidermal growth factor to EGFR induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation, differentiation, motility, and cell survival (1). Activation of EGFR triggers mitogenic signaling in gastrointestinal mucosa, and its expression is upregulated in colon cancers and most neoplasms (2). Activation of EGFR triggers activation of the ERK-signaling pathway in normal gastric epithelial and colon cancer cell lines. Inactivation of EGFR with selective inhibitors significantly reduces ERK2 activation, c-fos mRNA expression and cell proliferation.
Description: Recombinant Human EGFR also known as ERBB, mENA, ERBB1, and HER1 with C775S, T790M, and L858R mutations. Genbank Accession NM_005228, a.a. 668-end, with an N-terminal GST-tag, expressed in Sf9 cells via a baculovirus expression system. MW = 100 kDa.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Genbank: NM_005228
Storage Stability: At least 6 months at -80°C. Avoid freeze/thaw cycles.
Tags: N-terminal GST-Tag
Uniprot: P00533
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Wang, K., et al., J Biol. Chem 2004;279:53848-53856.2. Kobayashi, S., et al., New Eng. J. Med. 2005;325:786-792.
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