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Encompassing Amino Acids: 668-1210(end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay was done in Kinase buffer containing 1 mM DTT using Poly-(Glu4:Tyr) substrate (0.2 mg/ml) and 20 µM ATP. Reaction was done at 30°C for 45 minutes. Amount of ATP transferred was calculated using Kinase-Glo™ reagent (Promega).
Background: EFGR (epidermal growth factor receptor), also known as ErbB-1 and HER1, is the cell-surface receptor for members of the epidermal growth factor family, such as TGFα (transforming growth factor alpha) and belongs to the ErbB family of receptors. Overexpression and/or hyperactivation of EGFR is associated with several human cancers such as lung, glioblastoma, and epithelial tumors of the neck and head, whereas EGFR deficiency is linked to Alzheimer’s. After binding to its ligand, EGFR dimerizes and undergoes auto-phosphorylation, resulting in activation of signal transduction cascades that involve proteins such as JNK (c-jun N-terminal kinases) and AKT (protein kinase B). Its role as an oncogene has led to the development of inhibitors as cancer therapy.
Description: Recombinant human EGFR (epidermal growth factor receptor) encompassing tyrosine kinase domain amino acids 668-1210(end) with a deletion of amino acids 746 to 750 and mutations T790M and C797S. This construct contains an N-terminal GST-tag and a C-terminal His-tag (6xHis). This protein was affinity purified.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, and 3 mM DTT
Genbank: NM_005228
Host Cell Line: Sf9 cells
Purity: ≥65%
Storage Stability: At least 6 months at -80°C. Avoid freeze/thaw cycles.
Tags: N-terminal GST-tag, C-terminal His-tag
Uniprot: P00533
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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