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Encompassing Amino Acids: 668-1210
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay was done in Kinase buffer containing 0.2 mM DTT using Poly- (Glu4:Tyr) substrate (0.2 mg/ml) and 20 μM ATP. Reaction was done at 30°C for 40 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).
Description: Human EGFR T790M,C797S, L858R (GenBank Accession No.NM_005228), (a.a. 668-1210) with NterminalGST tag, MW = 88 kDa,expressed in baculovirus expressionsystem.
Format: Aqueous buffer solution
Formulation: 40 mM Tris pH 8.0, 110 mMNaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mMDTT and 20% glycerol
Genbank: NM_005228
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag, C-terminal His-Tag
Uniprot: P00533
Warnings: Avoidfreeze/thaw cycles. Storing diluted enzyme isnot recommended, if necessary, use carrierprotein (BSA 0.1 - 0.5%).
Biosafety Level: Not applicable (BSL-1)
References: 1. Thress, K.S., et al., Nat. Med. 2015; 21:560-562.
2. Niederst, M.J., et al., Clin. Canc. Res. 2015.
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