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Encompassing Amino Acids: 668-1210
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: The enzyme reaction was carried out for 1 hour at room temperature in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EDTA, 0.01% BRIJ-35 and 200 µM of ATP. Substrate : 2 µM Tyr peptide 4 from Invitrogen.
Description: Human EGFR T790M (GenBank Accession No. NM_005228), a.a. 668-1210 with substitution of Threonine790 to Methionine and an N-terminal GST-tag, MW= 88 kDa, expressed in a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.
Genbank: NM_005228
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag, C-terminal His-Tag
Uniprot: P00533
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Adachi,S. et al., Cancer Res. 67 (13), 6493-6501 (2007)
2. Rosano,L. et al., Cancer Res. 67 (13), 6351-6359 (2007).
Application Reference:
Figueroa, M., et al., J. Antibiotics. 65, 559-564 (2012).
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