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Applications: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Background: EHMT1 (GLP) is a histone methyltransferase that exhibits methylation activity toward H3-K9.
Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt
Description: The EHMT1 (GLP) Direct Activity Chemiluminescent Assay Kit is designed to measure EHMT1, also known as G9a-like protein or GLP activity for screening and profiling applications. The key to the EHMT1 (GLP) Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated K9 residue of H3. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primaryantibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
Storage Stability: 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Supplied As: The EHMT1 (GLP) Chemiluminescent Assay Kit comes in a convenient format, with a 96-well plate precoated with histone H3 peptide substrate, reagents, and purified EHMT1 for 100 enzyme reactions.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Huang, J., et al., J Biol. Chem. 2010 Mar 26; 285(13): 9636-41.
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