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Encompassing Amino Acids: 2-216
Applications: Suitable substrate for MNK1 kinase and other kinase assays.
Description: Human eIF4e (eukaryotic translation initiation factor 4E) (GenBank Accession No. NM_001968), a.a. 2-216 with N-terminal GST-tag, MW= 51 kDa, expressed in an E. coli expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione
Genbank: NM_001968
Storage Stability: >12 months at -80°C
Tags: N-terminal GST-tag
Uniprot: P06730
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Shih,J.W. et al., Oncogene 27(5), 700-714 (2008).
2. Salehi Z., Mashayekhi F. and Shahosseini F. Cell Biol. Int. 31(11), 1400-1404 (2007).Application Reference:MNK1-induced eIF-4E phosphorylation in myeloma cells: a pathway mediating IL-6-induced expansion and expression of genes involved in metabolic and proteotoxic responses (2014)