Target: ENaC.
Conjugate: APC .
Product Type: Polyclonal.
Immunogen: Produced against the C-terminal tail (amino acids 629-650) of rat gamma ENaC (antibody designation L550).
Swiss-Prot: P37091.
Purification: Protein A Purified.
Storage Buffer: PBS, 50% glycerol, 0.09% sodium azide.
Concentration: 1 mg/ml.
Specificity: Detects ~83kDa.
Cellular Localization: Apical Cell Membrane.
Scientific Background: The Epithelial Sodium Channel (ENaC) is a membrane ion channel permeable to Na+ ions. It is located in the apical plasma membrane of epithelia in the kidneys, lung, colon, and other tissues where it plays a role in trans epithelial Na+-ion transport (1). Specifically Na+ transport via ENaC occurs across many epithelial surfaces, and plays a key role in regulating salt and water absorption (2).ENaCs are composed of three structurally related subunits that form a tetrameric channel, α, β, and γ. The expression of its alpha and beta subunits is enhanced as keratinocytes differentiate (3, 4). The beta and gamma-ENaC subunits are essential for edema fluid to exert its maximal effect on net fluid absorption by distal lung epithelia(5). And it has been concluded that the subunits are differentially expressed in the retina of mice with ocular hypertension, therefore the up-regulation of alpha-ENaC proteins could serve as a protection mechanism against elevated intraocular pressure (6).
References: 1. Kakizoe Y., et al. (2009) J Hypertens. 27(8): 1679-1689.2. Gu Y. (2008) J Cell Physiol. 216(2):453-457.3. Bruns J.B. (2003) Am J Physiol Renal Physiol. 285(4): F600-F609.4. Mauro T., et al. (2002) J Invest Dermatol. 118(4): 589-594.5. Elias N., et al. (2007) Am J Physiol Lung Cell Mol Physiol. 293(3): L537-45.6. Dyka F.M., May C.A. and Enz R. (2005) J Neurochem. 94(1): 120-128.
Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
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